ALFREDO CRAVADOR

Professor Catedrático

  
Curriculum vitae
    

Nome: Alfredo Jaime Morais Cravador

    

Naturalidade: Santarém

    
   
Qualificações Académicas:

Agregação Ensino Superior - Universidade do Algarve - 1994

Doutoramento - Université Libre de Bruxelles (U.L.B.) - 1976

Licenciatura - Université Libre de Bruxelles (U.L.B.) - 1972
     
Actividades anteriores e situação actual em termos científicos e/ou profissionais:

Fevereiro de 1996 - pesente: Professor catedrático da Universidade do Algarve.

Junho de 1992 - Fevereiro de 1996: Professor associado da Universidade do Algarve, Agregado desde 7/1994.

Outubro de 1989 - Agosto de 1991: Consultor da sociedade SmithKline Beecham (Segurança Biológica dos Processos Biotecnológicos e Impacte Ambiental).

Outubro de 1988 - Setembro de 1989: Director científico da Empresa de Investigação e Desenvolvimento em Biotecnologia BIOEID.

1981 - 1988: Investigador na Université Libre de Bruxelles (U.L.B.).

1977 - 1981: Investigador assistente das Facultés Universitaires Notre-Dame de la Paix (Universidade de Namur).
     
Actuais interesses de investigação:

Domains and research programmes::

  • Study of the decline disease of Quercus suber and Quercus rotundifolia aiming at the management of the genetic/biological control of the disease. Studies on molecular interactions Phytophthora cinnamomi-Quercus suber in connection with the infectious process.

      •Identification and characterization of cork oak and holm oak trees tolerant to the decline disease associated with Phytophthora cinnamomi and evaluation of genetic and biological measures for its control.
      •The transcriptome analysis (ESTs) of Q. suber roots directed to genes related to biotic stress caused by Phytophthora cinnamomi infection.
      •Identification and production of active compounds against Phytophthora cinnamomi by Phlomis purpurea.
      •Metabolome and transcriptome analysis of Phlomis purpurea a plant resistant to Phytophthora cinnamomi.

  • Study of candidate genes as molecular markers in dairy indigenous species. Genetic and morphological characterization of indigenous bovine breeds.

 
     
Línguas:
  • Português (materna)
  • Francês
  • Inglês
  • Castelhano
   
Voltar ao topo
     
Publicações (mais relevantes):

  • M. Horta, P. Caetano, C. Medeira, I. Maia and A. Cravador. Involvement of the b-cinnamomin elicitin in infection and colonisation of cork oak roots by Phytophthora cinnamomi. European Journal of Plant Pathology 127 (3) Page 427-436 (2010). DOI: 10.1007/s10658-010-9609-x.

  • C. Ginja, M. C. T. Penedo, M. F. Sobral, J. Matos, C. Borges, D. Neves, T. Rangel-Figueiredo and A. Cravador. Molecular genetic analysis of a cattle population for recovery of the extinct Algarvia breed. Genetics Selection Evolution 42:18 (2010) doi: 10.1186/1297-9686-42-18.

  • H. R. Shahbazkia, M. Aminlari, A. Tavasoli, A. R. Mohamadnia and A. Cravador. Polymorphisms of the b-1,4 Galactosyltransferase-I Gene in Holsteins. Livestock Science 131 (2-3) Pages 297-30 (2010). doi: 10.1016/j.livsci.2010.04.009.

  • H. R. Shahbazkia, M. Aminlari, A. Tavasoli, A. R. Mohamadnia and A. Cravador. Associations among Milk Production Traits and Glycosylated Haemoglobin in Dairy Cattle; Importance of Lactose Synthesis Potential. Veterinary Research Communications, 34, 1-9, 2010, DOI 10.1007/s11259-009-9324-2.

  • M. F. Sobral, C. Roberto, D. Navas, I. Palmilha, M. B. Lima and A. Cravador. Identification of descendants of an extinct bovine population from the Algarve region of Portugal using numerical taxonomy analysis of morphological traits. Journal of Animal Breeding and Genetics, 126(4), 319-326 (2009).

  • Maia, I., Medeira, C., Candeias, I.; Moreira, A., Melo, E. and Cravador, A. Exposition of cork oak roots to cryptogein reduced the Infection by Phytophtora cinnamomi. Microscopy and Microanalysis, 15 (supp 3), 27-28 (2009).

  • M. Horta; N. Sousa; A. C. Coelho; D. Neves and A. Cravador. In vitro and in vivo quantification of elicitin expression in Phytophthora cinnamomi, Physiological and Molecular Plant Pathology, 73, 48–57 (2008).

  • Maia, I.; Medeira, C.; Melo, E.; Cravador, A. Quercus suber infected by Phytophthora cinnamomi. Effects at cellular level of cinnamomin on roots, stem and leaves. Microscopy and Microanalysis, 14 (supp 3), 146-147 (2008).

  • Barracosa P., Lima M.B., Cravador A. Analysis of genetic diversity in Portuguese Ceratonia siliqua L. cultivars using RAPD and AFLP markers. Scientia Horticulturae, 118, 189–199 (2008).

  • P. Barracosa, J. Osório, A. Cravador. Evaluation of fruit and seed diversity and characterization of carob (Ceratonia siliqua L.) cultivars in Algarve region, Scientia Horticulturae, 114, 250-257 (2007)

  • Coelho, A. C., Horta, M., Neves, D.  and Cravador, A. Involvement of a cinnamyl alcohol dehydrogenase of Quercus suber in the defence response to infection by Phytophthora cinnamomi. Physiological and Molecular Plant Pathology, 69 62–72 (2006).

  • Estela Bastos, Sílvia Ávila, Alfredo Cravador, Robert Renaville, Henrique Guedes-Pinto, José Luis Castrillo. Identification and characterization of four splicing variants of ovine POU1F1 gene. Gene, 382, 12-19 (2006).

  • Coelho, A.C.; Lima, M.B.; Neves, D. and Cravador, A. Genetic diversity of two evergreen oaks (Quercus suber L. and Q (ilex) rotundifolia Lam.) in Portugal using AFLP markers. Silvae Genetica, 55(3), 105-118 (2006).

  • M. R. Marques, I. C. Santos, N. Carolino, C. C. Belo, R. Renaville and A. Cravador. Effects of genetic polymorphisms at the growth hormone gene on milk yield in “Serra da Estrela” sheep. Journal of Dairy Research, 73, 394-405 (2006).

  • Estela Bastos, Ingrid Santos, Isabelle Parmentier, José Luis Castrillo, Alfredo Cravador, Henrique Guedes-Pinto, Robert Renaville. Ovis aries POU1F1 gene: cloning, characterization and polymorphism analysis. Genetica, 12(3): 303-314 (2006).

  • Maria Luisa Rodrigues, Margarida Archer, Paulo Martel, Sandra Miranda, Mónica Thomaz, Francisco Javier Enguita, Ricardo P. Baptista, Eduardo Pinho e Melo, Nelson Sousa, Alfredo Cravador, Maria Arménia Carrondo. Crystal structures of the free and sterol-bound forms of b-cinnamomin. Biochimica et Biophysica Acta - Proteins and Proteomics, 1764, 110-121 (2006).

  • Renaville R., Parmentier I., Falahi M., Gengler N., Cravador A. et Portetelle D. Les marqueurs génétiques. In Théwis A., Bourbouse A., Compère R., Duplan J.-M., Hardouin J. (eds).  Manuel de Zootechnie comparée Nord-Sud. INRA editions, Paris, 353-367 (2005).

  • P. X. Marques, M. Pereira, M. R. Marques, I. C. Santos, C. Belo, R. Renaville and A. Cravador. Association of milk traits with SSCP polymorphisms at the growth hormone gene in the Serrana goat. Small Ruminant Research, Vol 50/1-2, 177-185 (2003).

  • Maria L. Rodrigues, Margarida Archer, Paulo Martel,, Alain Jacquet, Alfredo Cravador and Maria A. Carrondo. Crystal structure of b-cinnamomin, a toxic protein for some plant species. Acta Crystallographica, D58, 1314-1321 (2002).

  • C. Reis and A. Cravador. Pit-1 gene HinfI polymorphism detection in eight portuguese bovine breeds. Rev. Port. Zootecnia, ANO IX - 1, 21-28 (2002).

  • M. F. Sobral, A.Cravador, D.Navas, C.Roberto, C.Reis, M.B.Lima (2001). Classification and morphological characterization of native Portuguese cattle breeds using numerical taxonomy. Rev. Port. Zootecnia, ANO VIII - 2, 123-137 (2001).

  • E. Malveiro, M.Pereira, P.X: Marques, I.C. Santos, C. Belo, R. Renaville and A. Cravador. Polymorphisms at the five exons of gowth hormone gene in the Algarvia Goat. Possible association with milk traits. Small Ruminant Research 41(2), 163-170 (2001).

  • C. Reis, D. Navas, M. Pereira, A. Cravador. Growth hormone AluI polymorphism analysis in eight Portuguese bovine breeds. Archivos de Zootecnia 50 (189-190), 41-48 (2001).

  • E. Malveiro, P. X. Marques, I. C. Santos, C. Belo and A. Cravador. Association between SSCPs at Algarvia goat GH and milk traits. Archivos de Zootecnia 50 (189-190), 49-57 (2001).

  • C. Bonifácio, I. Santos, C. Belo and A. Cravador. Single-Strand Conformation Polymorphism (SSCP) analysis of alfa s1-casein, beta-casein and kapa-casein genes in Charnequeira Portuguese indigenous goat breed. Archivos de Zootecnia 50 (189-190), 105-112 (2001).

  • Estela Bastos, Alfredo Cravador, Jorge Azevedo, Henrique Guedes-Pinto. Single strand conformation polymorphism (SSCP) detection in six genes in the Portuguese indigenous sheep breed "Churra da Terra Quente". Biotecnol. Agron. Soc. Environ., 5 (1), 7-15 (2001).

  • M. Archer, M. L. Rodrigues, M. Aurélio, R. Biemans, A. Cravador, M. A. Carrondo Crystallisation and preliminary X-ray diffraction analysis of beta-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi. D 56, 363-366 (2000).

  • J. Duclos, A. Fauconnier, A.C. Coelho, A. Bollen, A. Cravador and E. Godfroid. Identification of an elicitin gene cluster in Phytophthora cinnamomi; DNA Sequence 67, 231-237 (1998).

  • A. Cravador. Os DNA Sintéticos Anti-Sentido, Química Nova, 21 (4), 441 (1998).

  • A.C. Coelho, A. Cravador, A. Bollen, J.F.P. Ferraz, A.C. Moreira, A. Fauconnier and E.Godfroid. Highly specific and sensitive non-radioactive molecular identification of Phytophthora cinnamomi; Mycological Research, 101 (12): 1499-1507 (1997).

  • A. Cravador. Les oligonucléotides synthétiques “antisens” (1re partie); Chimie Nouvelle, 12(46), 1351 (1994).

  • A. Cravador. Les oligonucléotides synthétiques “antisens” (2e partie); Chimie Nouvelle, 12(47), 1377 (1994).

  • M.-J. De Vos, A. Cravador, J.-P. Lenders, S. Houard and A. Bollen. Solid Phase Non Isotopic Labelling of Oligodeoxynucleotides Using 5'-Protected Aminoalkyl Phosphoramidites: Application to the Specific Detection of Human Papilloma Virus DNA; Nucleosides & Nucleotides, 9(2), 259 (1990).

  • A. Cravador, A Herzog, S. Houard, P. d'Ippolito, R. Carrol and A. Bollen. Selective detection of Human Papilloma Virus DNAs by specific DNA probes; Molecular and Cellular Probes, 3, 143 (1989).

  • N. Moguilevsky, C. Roobol, R. Loriau. J.-P. Guillaume, P. Jacobs, A. Cravador, A. Herzog, L. Brouwers, A. Scarso, P. Gilles, L. Holmquist, L. A. Carlson and A. Bollen. Production of Human Recombinant Proapolipoprotein A-I in Escherichia coli: Purification and Biochemical Characterization; DNA, 8(6), 429 (1989).

  • M.Chaput, V.Claes, D.Portetelle, I.Cludts, A.Cravador, A.Burny, H.Gras, A.Tartar. The neurotrophic factor neuroleukin is 90% homologous with phosphohexose isomerase; Nature 332, 454 (1988).

  • H. Johansen, J. Sutiphong, G. Sathe, P. Jacobs, A. Cravador, A. Bollen, M. Rosenberg and A. Shatzman. High-level production of fully active human alpha1-antitrypsin in Escherichia coli, Mol. Biol. Med. 4, 291 (1987).

  • P. Jacobs, F. Brockly, M. Massaer, R. Loriau, J. P. Guillaume, E. Ciccarelli, M. Heinderyckx, A.Cravador, R. Biemans, A. Van Elsen, A. Herzog, A. Bollen. Porcine D-amino acid oxidase: determination of the mRNA nucleotide sequence by the characterization of genomic and cDNA clones; Gene, 59, 55 (1987).

  • Mutant and chimaeric recombinant plasminogen activator: production in eukariot cells and preliminary characterization; J. Biol. Chem., 262(24), 11771 (1987).

  • Full-length sequence and expression of the 42 kDa 2-5a synthetase induced by human interferon; FEBS Lett. 196, 113 (1986).

  • Molecular cloning, full-length sequence and preliminary characterization of a 56 kDa protein induced by human interferon; Eur. J. Biochem., 155, 11 (1986).

  • A.Cravador, P.Jacobs, A.Van Elsen, C.Lacroix, B.Colau, P.Van Alphen, A.Herzog, A.Bollen.Total DNA synthesis and cloning in Escherichia coli of a gene coding for the human growth hormone releasing factor; Biochimie, 67, 829 (1985).

  • Synthesis of selenoacetals; Tetrahedron, 41(21), 4793 (1985).

  • Molecular cloning sequencing and expression in Escherichia coli of human preprourokinase cDNA; DNA, 4(2), 139 (1985).

  • A. Cravador. A síntese química de ADN - Um instrumento indispensável às manipulações genéticas; Química Nova, 8(2), 105 (1985).

  • A. Cravador. La synthèse chimique de ADN. Un outil indispensable aux manipulations génétiques; Chimie Nouvelle, 2 (6), 71 (1984).

  • Cloning and expression in Escherichia coli of full-length complementary DNA coding for human alpha1-antitrypsin; DNA, 2,(4), 255 (1983).

  • Gene activation and re-expression of a Trypanosoma brucei variant surface glycoprotein; The EMBO Journal, 2 (7), 1185 (1983).

  • Unusual reactivity of selenoboranes towards epoxides: new selective routes to hydroxyselenides and allylalcohols; Tetrahedron Letters, 22, 2491 (1981).

  • Nouvelles méthodes de synthése utilisant une entité séléniée comme précurseur de fonction; Bull. Soc. Chim. France II 519 (1980).

  • Unexpectedly easy formation of alpha-seleno carbonyl compounds from vinyl selenides, J.C.S. Chem. Comm.;951 (1980).

  • Reduction vs acetalisation of some carbonyl compounds by means of methylselenol and Lewis Acids; J.C.S. Chem. Comm., 451 (1980).

  • Nouvelles méthodes de synthèse des méthylsélénoacétals et cétals; C.R.Acad.Sc.Paris; 289, 267 (1979).

  • 1,1-bis(phenylseleno) and 1,1-bis(methylseleno)alkyllithium as building blocks in organic synthesis, J.Organomet.Chem.;177, 1 (1979).

  • Deprotection of selenoketals to carbonyl compounds A comparative study, Synthesis, 877 (1979).

  • Acidité relative des groupes méthyle de la 2,4,6-collidine dans le système phényllithium-solvants éthérés; Rev. Port, Quím., 20 , 65 (1978).

  • Chemical ecology of Arthropods IX. Stucture and absolute configuration of hippodamine and convergine, two novel alkaloids from the american ladybug Hippodamia convergens; Tetrahedron Letters, 5, 409 (1974).

  • Two novel alkaloids from the american ladybug Hippodamia Convergens; Bull. Soc. Chim. Belges, 81, 649 (1972).

     

Voltar ao topo
     
 
     
 
     
Voltar ao topo
     
     
 
     
 
 
     
Contactos

Alfredo Jaime Morais Cravador
Universidade do Algarve
Faculdade de Ciências e Tecnologia
Laboratórios de Biotecnologia Molecular e Fitopatologia (D2,D3,G2,G3 e G4)
Campus de Gambelas
8005-139 FARO
PORTUGAL


Telf.: (+351) 289 800 935
GSM: (+351) 934 212 286
  Fax.: (+ 351) 289 800 066
     

Voltar ao topo